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WebFeb 2, 2024 · Apoptotic cells were quantitated using the Annexin V-FITC/propidium iodide (PI) apoptosis assay kit (KeyGEN BioTECH, China). Briefly, PC12 cells were digested with 0.25% trypsin, centrifuged, and resuspended in 200 μL 1 × binding buffer. Then, cells were stained with 5 μL Annexin V-FITC in the dark for 10 min. WebFITC is a fluorescein derivative consisting of a fluorescein core and an amine-reactive isothiocyanate moiety. The isothiocyanate group reacts with primary amines forming … inch frisør st hanshaugen
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